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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Selective Targeting of Human and Animal Pathogens of the Helicobacter Genus by Flavodoxin Inhibitors: Efficacy, Synergy, Resistance and Mechanistic Studies
doi: 10.3390/ijms221810137
Figure Lengend Snippet: Thermodynamic analysis of the interaction between compound IV and wild-type Hp -Fld and mutants N14A, V113W, Q115W, T116W and K133A by ITC. ( A ) The upper plots show the thermograms (thermal power as a function of time), whereas the lower panels exhibit the binding isotherms (titrant normalised heat effects as a function of the ligand:protein molar ratio in the cell). In these last ones, the solid line corresponds to the best fit. ( B ) Thermodynamic parameters of each flavodoxin interaction with compound IV. Gibbs energy (Δ G ), enthalpy (Δ H ) and entropic contribution (− T Δ S ) are represented in black, grey and white bars, respectively.
Article Snippet: Thermodynamic parameters of the binding equilibrium were calculated by non-linear least-squares regression analysis considering a single ligand binding site and using the user-defined fitting routines in the
Techniques: Binding Assay